Phenylalanine hydroxylase deficiency

(PAH deficiency)
  • Clinical

    Untreated: severe brain damage with intellectual disability, seizures, spasticity, movement disorder.
    Treatment does not generally reverse neurologic symptoms that have already occurred.

  • Variants

    • PKU: requires diet (differences in disease severity/Phe tolerance: severe PKU = homozygous null mutation, mild PKU = at least one hypomorphic/residual function mutation)
    • MHP: mild hyperphenylalaninemia, does not require diet therapy (Phe <600 µmol/l in Germany, <400 µmol/l in the UK, <360 µmol/l in the USA)
    • BH4-responsive PKU: reduction of Phe levels after BH4 supplementation in many patients with mild PKU (stabilization/activation of mutant protein).
  • Genetics

    Loss of function effect, >1100 pathogenic variants with different residual activities, genotype may allow prediction of severity and BH4 responsiveness. PAH mutation database: https://www.biopku.org 

  • Incidence

    In Europe up to 1:4,400 (Ireland), average ~1:8,000

  • Diagnosis, Investigations

    Newborn screening (filter paper card), AA (plasma): ­ ↑Phe, n–↓Tyr

  • Differential diagnosis

    Disorders of tetrahydrobiopterin (BH4 cofactor) metabolism; DNAJC12 deficiency

  • Treatment

    Phe-restricted diet, supplementation of essential amino acids + micronutrients. 
    BH4 supplementation in mild PKU with BH4 responsiveness (not all patients).
    Exact recommendations differ between countries: 

    • D/A/CH: treatment if Phe > 600 µM, target range age 0-6: 40–240 µmol/l, 
      age 7-12: 40-360 µmol/l, age 12-18: 40-600 µmol/l; adult 40-1200 µmol/l
    • UK: treatment if Phe > 600 µM, 
      target range: 120-360 µmol/l, school-age or older: 120-480 µmol/l
    • USA: treatment if Phe > 360 µM, target range 120–360 µmol/l throughout life
    • “European” (ESPKU): treatment if Phe > 360 µM, 
      target range age 0-12: 120–360 µmol/l, from age 12 incl. adults: 120–600 µmol/l
  • Prognosis

    Normal development and intelligence with immediate and efficient treatment.